Fishery: long shell in summer, long flesh in autumn. In the cool autumn season, it is the peak season for fish growth. The following four levels must be considered: Magnetic Bead Nucleic Acid Extraction Reagent
The magnetic bead method nucleic acid extraction kit is a high-tech product that combines biological science and nanomaterial science. It is a major breakthrough in my country's nucleic acid extraction and purification technology. .
Magnetic bead method for nucleic acid extraction has incomparable advantages over traditional DNA extraction methods, which are mainly reflected in: 1. It can realize automatic and large-scale operation. At present, there are 96-well nucleic acid automatic extraction instruments, and the extraction time of one sample can be realized. The processing of 96 samples complies with the high-throughput operation requirements of biology, enabling rapid and timely response to infectious disease outbreaks, which makes traditional methods unmatched; â‘¡ The operation is simple and time-consuming, and the entire extraction process has only four steps , most of them can be completed within 36-40 minutes; â‘¢ Safe and non-toxic, no toxic reagents such as benzene and chloroform in traditional methods are used, and the damage to experimental operators is minimized, which fully conforms to modern environmental protection concepts; â‘£ Magnetic beads and nucleic acids The specific binding of the extracted nucleic acid results in high purity and high concentration of the extracted nucleic acid.
According to the same principle as the silica membrane spin column, the superparamagnetic silica nano-magnetic beads are prepared after the surface of the superparamagnetic nanoparticles is modified and modified by nanotechnology. The magnetic beads can specifically recognize and efficiently bind to nucleic acid molecules on a microscopic interface. Using the superparamagnetic properties of silica-coated nanomagnetic microspheres, under the action of Chaotropic salts (guanidine hydrochloride, guanidine isothiocyanate, etc.) and an external magnetic field, samples from blood, animal tissues, food, pathogenic microorganisms, etc. The isolated DNA and RNA can be used in clinical disease diagnosis, blood transfusion safety, forensic identification, environmental microbial testing, food safety testing, molecular biology research and other fields.
Magnetic bead nucleic acid extraction can generally be divided into four steps: lysis-binding-washing-elution.
Genetic testing will surely become a new symbol of the development of the biological industry. The emergence of high-throughput, automated nucleic acid extraction methods will reduce the labor cost of genetic testing, make large-scale testing a reality, and make it possible for genetic testing to reach ordinary people.
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One, put a good feeding and fertilizer off.
Generally, fertilization and feeding amount in September to November should account for about 30% of the total annual fertilization and feeding amount. When applying organic fertilizer, it should be a small number of times, so as to avoid over-flooding caused by pond fish floating head; application of inorganic fertilizer, per acre can use carbon ammonia 5 ~ 7.5 kg, or urea 2.5 ~ 3.5 kg. After the “white dewâ€, the resources of grasses gradually decreased. At this time, concentrate feed should be the main method. ?
Second, turn off the management of good water quality. After the fall, the temperature dropped, and the amount of bait in the fish ponds was relatively increased. Therefore, the water should be changed frequently to keep the fish ponds fresh and fat and not dirty.
Third, to prevent fish floating head off.
For individual fish ponds with large intensive culture densities, due to the large amount of fertilizer applied for feeding, the organic matter content in the water is large, and the oxygen consumption factor is also increased, so that the phenomenon of anoxic floating heads is prone to occur. You can turn on the aerator in the evening or early morning, or add new water to adjust.
Fourth, the prevention and control of good fish disease.
After the "White Dew", although the fish disease season has passed, it must not be ignored. From September to November, every 15 to 20 days, fish ponds with a depth of 1 meter per acre need to be sprayed with quick lime 15 to 20 kilograms each time, and 300 grams of bleaching powder can also be used to disinfect the fishpond bags.