Nature shows: CRISPR/Cas9 finds new targets for treating AIDS

A study published online by the British journal Nature Genetics on the 19th showed that American scientists using CRISPR/Cas9 gene editing technology have discovered novel genes that are indispensable for human immunodeficiency virus (HIV) infection and can prevent HIV infection. But it does not affect cell viability, making them a promising candidate for drug or gene therapy.

HIV, the HIV virus, is the cause of AIDS. By destroying the body's T lymphocytes, it blocks the cellular and humoral immune processes, causing the immune system to paralyze, causing various diseases to "ravage" in patients, eventually leading to AIDS. Due to the extremely rapid variation of HIV, it is difficult for humans to develop specific vaccines, so there is no effective treatment method to date, which poses a great threat to human health throughout the world.

CRISPR/Cas9发现治疗艾滋病新靶点

This time, the Whitehead Biomedical Research Institute, the Harvard-MIT Institute of Bode Research, and the Massachusetts General Hospital Lagan Institute collaborated to establish a T cell bank using the CRISPR/Cas9 method. T cells are the natural host of HIV. In this T cell bank, most of the genes in the human genome are first inactivated one by one (a type of gene therapy strategy that interferes with certain pathogenic genes at the level of DNA or mRNA). Transcription, translation, etc. in normal expression, thereby blocking the expression and function of the gene). Subsequently, the research team infects these cells with HIV and screens for genes that mutate to make cells immune to HIV infection without affecting normal cell function.

In the study, the team found a total of five such genes, two of which are known to be closely related to HIV infection; three new genes are outstanding and are isolated in subsequent functional experiments and from healthy human donors. Their findings were verified in the T cells.

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