Seed SSR Detection - High-throughput Pre-Processing Solutions

US SPEX SamplePrep has more than 60 years of professional sample preparation equipment production experience and professional background. The SPEX GENO high-throughput tissue grinder was originally developed specifically for DuPont's pioneering PIONEER plant samples.

SSR (also known as microsatellite DNA) markers, due to their codominant genetic characteristics, can identify heterozygotes and homozygotes. The labeling method requires less DNA, good experimental repeatability, high reliability, simple and economical operation, and thus has become the most commonly used molecular identification method in seed identification, and has appeared in some standards for seed authenticity and purity detection.

In order to achieve extremely high throughput detection, it would be ideal to successfully pulverize a single seed in a single well of a 96-well deep well plate. We know that it is not difficult to grind seeds, but it is not so easy to grind hard seeds in such a small space. The SPEX high-throughput tissue grinder can easily do this. Pei'an application engineers have compiled the best pre-treatment options for hundreds of samples. The figure below shows the grinding effect of some seeds (each of which is the result of a single-hole grinding single seed in a 96-well deep well plate):

It is worth noting that the skinned seeds of barley, mung bean and soybean do not need to be peeled, and the outer skin naturally peels off after grinding; the hard seeds like soybeans are successfully crushed in a single hole; oily seeds like sunflower seeds, although the results of grinding Some agglomerates, but also muddy, with good fineness. The corn seed alone is large and cannot be placed in a single well of a deep well plate, but when we put the corn seed into a 15 ml tube, we also obtained the grinding effect of the following figure:

corn

We know that in the detection of seed authenticity and purity, each variety needs to be repeated in a certain amount, especially in the purity test, at least 100 seeds are needed. In the first step of the SSR molecular calibration step, it is necessary to break the seeds and extract the DNA, which is a very heavy manual labor. If SPEX Geno joins the job, you can get the template DNA of all the seeds to be tested in a matter of minutes! And this is often the amount of labor that requires a few working days for a person!

Geno 2010 can process 6 96-well deep-well plates at a time, which means that 576 seeds can be extracted at one time; 24 15ml tubes can be processed at one time, and large particles like corn can be used 24 times at a time. DNA extraction of the sample.

The high sample throughput in the field of seed detection and genetic breeding is well known and a problem for researchers. Application engineers at the Pei'an Technology Center can provide you with solutions based on your samples, so that all the cumbersome pre-processing problems can be solved.

We hope that Pei's professional pre-treatment equipment and technical services will help you to make your research career as soon as possible.

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