The use of a one-time mushroom cultivation method for cultivation of mushroom, the cultivation cycle is about 40 days shorter than the traditional cultivation, economic efficiency can be increased by 78%, and the number of mushrooms increased, the shank increased, the quality of the goods greatly improved. The technical points are as follows: 1. According to the ratio of cottonseed hull 84%, wheat bran 14%, gypsum 1%, potassium dihydrogen phosphate 0.20%, magnesium sulfate 0.25%, and carbendazim 0.55%, the culture materials were mixed, and water was added to the culture materials. The ratio is 1:1.3, mix well and put it in a wide-mouth bottle (bag). 2. After the cultivating material is bottled (bag), the bottle (bag) port is sealed, placed in an oven and sterilized, and cooked at 100°C for 8 to 10 hours. If using a pressure cooker to sterilize, cooking can continue for 90 minutes under a pressure of 1.5 kg/cm2. 3. After the culture material to be sterilized is cooled, under the flame of the alcohol lamp in the inoculation room or in the inoculation box, use the inoculum shovel to dig out the bacteria seed block from the strainer bottle, move it into the culture bottle (bag), and put it in In the culture room, hypha culture was performed at room temperature of 22-26°C. 4. After the mycelium grows over the bottle (bag), the bottle (bag) is moved into the mushroom farm for mushroom culture. After that, the bottle (bag) is opened for mushroom germination. 5. After the buds of Flammulina velutipes are formed, they should be bagged and covered with newspapers to promote the formation of a microclimate with a high concentration of carbon dioxide in the bottles (bags). 6. When the mushroom grows to 4-6 cm, it should be subject to moisture management. At the same time, the temperature should be maintained at 8-14°C, and cover the light with a straw or black plastic cloth in the cultivation site. The light intensity can be blurred with normal vision. The Chinese characters on the newspaper are appropriate. When the mushroom grows to 15-20 cm, it can be harvested. Coaxial Breathing Systems,Pediatric Circle Breathing Systems,Medical Anaesthesia Machine Accessories,Anesthesia Machine Breathing Circuit Accessories Zhejiang Haisheng Medical Device Co., Ltd , https://www.hisernmedical.com