A new breakthrough in disease detection! Nano-enzyme test strips increase sensitivity by a factor of 100

Release date: 2015-10-21

The Ebola outbreak in West Africa in 2014 shocked the world. In the absence of effective treatment at the time, people quickly realized the importance of early detection and isolation of patients to control the epidemic. Although technologies such as RT-PCR and ELISA have played an important role, these technologies are difficult to be rapidly popularized due to local environmental constraints. In view of this, "Nature" even specifically called on scientists to develop new detection techniques that are simple, fast and economical.

Based on the work of nano-enzymes (NatureNanotechnology 2007, 2012), the research group of the Institute of Biophysics of the Chinese Academy of Sciences has developed a new technology for “nano-enzyme test strips”. This technology replaces the colloidal gold in the traditional test strip with magnetic nano-enzyme, which improves the sensitivity of the test strip by 100 times. The mechanism of this signal amplification comes from two aspects: First, the nano-enzyme probe molecule has magnetic properties. The sample can be separated and enriched; secondly, the nano-enzyme has catalytic activity, can make the substrate color, and improve the detection sensitivity. This new technology not only provides a simple means for rapid detection of Ebola and other viruses, but also can be applied to field investigations such as early diagnosis of tumors and inspection and quarantine of customs ports at customs by switching the types of antibodies on the probes.

In collaboration with Prof. Gao Fu, Research Fellow of the Institute of Microbiology, Chinese Academy of Sciences, and researcher Cao Wuchun from the Academy of Military Medical Sciences, Yan Xiyun's research team successfully applied the technology to the detection of Ebola, Newbuna, and influenza. At present, this result has been published in the bio-detection professional journal "BiosensorsandBioelectronics", and was immediately rated as a ScienceDirect hot article (Top25HottestArticles); ELSEVIER also distributed a special comment, saying that the technology overcomes the traditional test strip method The bottleneck problem of limited long-term application due to low sensitivity is expected to become a weapon for controlling Ebola transmission.

Source: Jianke Net

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