1. Remove the required slats from the foil pouch after equilibrating for 20 min at room temperature. The remaining slats are sealed back to 4 °C with a ziplock bag. 2. Set standard and sample wells, and add standard concentration of 50μL to each standard. 3. Add 50 μL of the sample to be tested to the sample well; blank holes are not added. 4. In addition to the blank wells, add 100 μL of horseradish peroxidase (HRP)-labeled detection antibody to each well of the standard wells and sample wells, seal the wells with a sealing membrane, and incubate in a 37 ° C water bath or incubator. 60min. 5. Discard the liquid, pat dry on the absorbent paper, fill each well with the washing solution (350 μL), let stand for 1 min, remove the washing solution, pat dry on the absorbent paper, and repeat the washing 5 times (can also be washed with a washing machine) board). 6. Add 50 μL of substrate A and B to each well and incubate at 37 ° C for 15 min in the dark. 7. Add 50 μL of stop solution to each well, and measure the OD value of each well at a wavelength of 450 nm within 15 min. Experimental principle The kit uses a double antibody one-step sandwich enzyme-linked immunosorbent assay (ELISA). To the coated microwells pre-coated with human metallothionein (MT) capture antibody, specimens, standards, HRP-labeled detection antibodies were sequentially added, incubated and thoroughly washed. Using the substrate TMB to develop color, TMB is converted to blue under the catalysis of peroxidase and converted to the final yellow color by the action of an acid. The color depth is positively correlated with human metallothionein (MT) in the sample. The absorbance (OD value) was measured at 450 nm using a microplate reader to calculate the sample concentration. Remarks: 1. The concentration of standard products is: 2400, 1200, 600, 300, 150, 0 pg/mL 2. After a large number of normal specimen tests, the normal concentration values ​​of the specimens are within the detection range provided by the kit, and 50 μL of the sample can be directly loaded during the experiment. When part of the sample value exceeds the maximum standard concentration, the sample may be diluted with the sample dilution before the experiment. Grain powder is a kind of solid powder obtained by spray drying after breaking the wall of grain with high protein content. It can also be made into small molecular peptides by enzymolysis. It is a good helper for food additives and nutritional supplements. Anthocyanin Extract，Corn Extract，Dog Rose Extract Fufeng Sinuote Biotechnology Co.,Ltd. , https://www.sntextract.com
Human metallothionein (MT) ELISA kit
Type of experiment: Sandwich method detection range: 75 pg/mL – 2400 pg/mL
Minimum detection limit: 10 pg/mL
Applications: Serum, plasma, tissue homogenate, cell culture supernatant or other related fluids (this product is for laboratory research and non-clinical use only) 
Steps Kit composition 96-well configuration 48-hole configuration Remarks Microporous plate 8 holes × 12 8 holes × 6 no Standard 0.3mL×6 tube 0.3mL×6 tube no Sample diluent 6mL 3mL no Detection antibody-HRP 10mL 5mL no 20× washing buffer 25mL 15mL Dilute according to the instructions Substrate A 6mL 3mL no Substrate B 6mL 3mL no Stop solution 6mL 3mL no Sealing film 2 sheets 2 sheets no Instruction manual 1 serving 1 serving no Ziplock bag 1 1 no
Detection principle and operation method of metallothionein content detection kit (human source)
Detection principle and operation method of metallothionein content detection kit (human source)
English name: Human MT ELISA Kit