How to check the titer of the tag antibody? KT3 epitope tags are often recombined into the N-terminus or C-terminus of the target protein for visual observation and localization using practical immunohistochemistry or fluorescence chemistry techniques. The amino acid sequence of the KT3 tag is KPPTPPPEPET. The Abbkine-specific KT3 monoclonal antibody recognizes a KT3 fusion protein derived from the simian virus 40 large T antigen (SV40) sequence. How to choose the right KT3 tag antibody? When choosing KT3 antibodies, we mainly need according to our own needs, especially for experimental applications. In addition, the more types of applications after an antibody is detected, the greater the choice in use; while the monoclonal antibodies from mouse sources generally have better specificity, stability, and potency than polyclonal antibodies. In addition, the titer of the antibody is also an important aspect to examine the cost performance of the antibody, which is equivalent to the dilution ratio in practical use. A high titer of 100 μl KT3 antibody (if the dilution ratio of WB is 1:5000, the final solution is equivalent to 500 ml), 1 ml of KT3 antibody with lower potency (if the dilution ratio of WB test is 1:200) The final use solution is equivalent to 200ml). Why recommend the Abbkine brand KT3 tag antibody (clone number 14D8 )? At present, the KT3 tag antibody available on the market has been domestically packaged or produced. The domestically packaged KT3 tag antibody has insufficient quality stability, and its valence is also lost after being packaged. Some KT3 tag antibodies originating in domestic laboratories have better detection activity and are difficult to control in batch stability. However, only a small number of native antibodies in China are also polyclonal antibodies. As for the imported brands that supply KT3 tag antibodies, there are not many imported brands. Only Abcam, Sigma and other brands can provide KT3 tag antibodies, but they are far less expensive than Abbkine products. "Comprehensive comparison of KT3 tag antibodies" Therefore, we recommend Abbkine's Anti-KT3 Tag Mouse Monoclonal Antibody (14D8). Like Abcam, the immunogen of Abbkine's KT3 antibody is a synthetic KT3 polypeptide. After continuous screening and optimization, the best cell line is finally obtained. 14D8). As a production brand in the United States, Abbkine provides OEM services to a number of internationally renowned brands, so its price is low and its quality has undergone market testing. This antibody can be used for WB. Antibody verification results (A, 1:3000 dilution; B, 1:5000 dilution) Abbkine also offers 1ml large packaging and larger custom-made KT3 antibodies, making it ideal for routine KT3 immunological assays. For the convenience of customers, Abbkine is available in stock in China. Abbkine provides global product quality tracking and after-sales service and protection for the Chinese market. If you have any problems during use, please contact Abbkine local agent or Aimei Technology Co., Ltd. directly. The American Abbkine brand is a brand that provides cost-effective immunology and cytology research products and solutions. Amy Jie introduced it to China as its regional agent in China, and will provide customers with the most cost-effective routine immunological reagents, such as A tag antibody, an internal reference antibody, a conventional primary antibody, and a full range of secondary antibodies, antibody detection substrates, and related kits. At the same time, as a Chinese agent or regional agent of many international famous brands, Aimei can provide you with a series of laboratory solutions. If you are interested in the above products, please call the free customer line 400-6800-868 to contact Aimei Technology Co., Ltd. for product information and complete experimental solutions. COVID-19 Infectious Disease Test Kit Corona viruses are enveloped RNA viruses that are distributed broadly among humans,other COVID-19 infectious diseases test Kit NINGBO AUTRENDS INTERNATIONAL TRADE CO., LTD , https://www.metests.com Product name and description Item number product manual Anti-KT3 Tag Mouse Monoclonal Antibody (14D8) A02110 Details Brand Item number App types Price / specification Abbkine A02110 WB: (1:5000) 605/50ul 1800/200ul 6020/1ml Abcam Ab3859 WB: (1:1000-1:10000) 3996/100ul Product name and description Item number product manual Anti-KT3 Tag Mouse Monoclonal Antibody (14D8) A02110 Details
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