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Both bottled purified water and distilled water are ordinary pure water instead of high-purity water. The water still contains a small amount of impurities such as ions and organic substances. The liquid phase and eluent can not only foul the expensive column, but also affect the HPLC to determine the flat baseline. .
The purification process of bottled purified water usually includes adsorption, filtration, reverse osmosis, etc., and the removal of particulate organic matter is better, but the removal of trace organic matter cannot meet the requirements of high sensitivity HPLC experiments, and, because of its packaging, usually Mostly PVC bottles, high oxygen permeability, and a certain amount of chemical dissolution, thus polluting the pure water in the bottle, especially after a period of storage, the water quality drops more, so it is expressed on the HPLC chromatogram, although there is no specific absorption Peak, but there is a serious instability disturbance at the baseline.
Some experts have found that the 246nm and 214nm have strong absorption peaks at 22-27 minutes, which indicates that there is a strong hydrophobic organic matter, which is caused by the azeotropic phenomenon of the distillation process. This has led to incomplete removal of certain volatile organic compounds.
After the ultra-pure water system integrates multiple processes such as water quality pretreatment, two-stage reverse osmosis, atomic ultra-purification, and high-precision ultrafiltration, the water quality index can be compared with the imported ultra-pure water machine, and the ultra-pure water is taken immediately. It will not be polluted by storage, and the water quality is guaranteed. Compared with the use of bottled purified water or distilled water, it can meet the needs of users for high-precision instrumental analysis.
(Note: The higher the purity of the water, the more susceptible it is to external pollution caused by air and containers. Ultrapure water should be used immediately. In this way, the influence of external disturbances can be eliminated as much as possible in high-precision instrumental analysis. Thereby obtaining satisfactory results.)
Effect of ultrapure water on high performance liquid chromatography (HPLC) experiments
With the development of the times and technology, precision analytical instruments such as high-performance liquid phase have been popularized in industries related to analytical testing, and the requirements for experimental water quality are also increasing. Today's high performance liquid chromatography (HPLC) requires users to use ultrapure water to set a flat baseline without peaks to ensure the sensitivity of the analytical test, but some HPLC users still use bottled drinking pure water or distilled water for experiments due to funds or habits. The effect is often not ideal.