The Thermal Cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR).[1] Thermal cyclers may also be used in laboratories to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.[2] The device has a thermal block with holes where tubes holding the reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps.
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Special attention is paid to the fact that in addition to controlling the temperature inside the shed, "high-temperature melons" must maintain high humidity and sufficient carbon dioxide. When carrying out the “high temperature for melonâ€, appropriate ventilation should be carried out under the premise of ensuring proper temperature. When the contradiction between heat preservation and ventilation cannot be solved, carbon dioxide gas fertilizer can be used to solve the problem.
"High temperature melon" should have degree
The so-called “high-temperature melon cultivation†refers to the fact that the carbon dioxide concentration in the shed is sufficient. The higher the temperature, the higher the photosynthetic efficiency of the plant and the more nutrition the melon strip can obtain. In the period of cucumber melon period, the temperature in the shed is controlled slightly higher, which is beneficial to the growth and development of melon strips. However, if the temperature is too high, it will affect the growth of cucumber. When the greenhouse temperature reached 38 °C, the photosynthetic efficiency of the plants was the highest, but the growth of plants exceeded 40 °C, and if the temperature exceeded 40 °C, the plants showed wilting symptoms. Therefore, the temperature in the shed should be changed to 38°C in management, and the temperature in the ventilation shed can reach 35°C.