Introduction to the prolonged life-related precautions during pipette use

The pipette is a precision instrument consumable . It should be operated strictly in accordance with the precautions during use to minimize errors and prolong service life. So what are the precautions related to pipettes? Let's take a look together.

Precautions for extending the life of the pipette during use :

1. Set the pipetting volume

Adjusting from a large range to a small range is a normal adjustment method, and rotating the scale counterclockwise

When adjusting from small range to large range, it should be adjusted to exceed the set volume scale and then adjusted back to the set volume, which can ensure the accuracy of the pipette.

2. Assembly pipette tip

Insert the pipette vertically into the tip, rotate it a half turn to the left and right, and tighten it.

It is very unacceptable to use a pipette to hit the tip. This long-term operation causes the parts of the pipette to loosen due to impact, which can cause the knob of the adjustment scale to jam.

3. Aspiration and drainage

Vertical pipetting

The tip of the tip is immersed in the liquid surface below 3mm, and the tip of the tip is pre-washed in the liquid before aspiration.

Slow suction slow release

If the amount is small when draining, the tip of the tip of the tip should be the inner wall of the container.

4. The pipette that sucks the liquid should not be laid flat. The liquid inside the pipe tip can easily contaminate the inside of the gun and may cause the spring of the gun to rust.

5. The pipette should adjust the scale to the maximum after each experiment, and let the spring return to the prototype to extend the life of the pipette.

6. When sucking the liquid, be sure to release the thumb slowly and smoothly. Never let it suddenly loosen, in case the solution is sucked too fast and flush into the liquid extractor to corrode the plunger and cause air leakage.

7. In order to obtain higher precision, the sample solution needs to be sucked once before the sample is taken, and then the liquid is officially pipetted. When the serum protein solution or organic solvent is sucked, a “liquid film” remains on the inner wall of the tip, causing the liquid discharge amount. It is too small and produces errors.

8. For liquids with large concentrations and viscosities, errors will occur. To compensate for the error, the amount of compensation can be determined by experiment. The amount of compensation can be set by changing the reading of the reading window with the adjustment knob.

9. The weight of the pure water taken by the analytical balance can be weighed and calculated to correct the liquid take-up. 1 mL of distilled water weighs 0.9982 g at 20 °C.

10 When setting the range, please note that the rotation to the required range is clear. In the display window, the set range should not be rotated out of the range within the range of the pipette. Otherwise, the mechanism will be stuck and the pipetting will be damaged. Device.

11. Pipettes are strictly prohibited to absorb liquids with strong volatility and strong corrosiveness (such as concentrated acid, concentrated alkali, organic matter, etc.).

12. Do not use a pipette to blow the mixed liquid.

13. Do not use a large-scale pipette to remove a small volume of liquid, so as not to affect the accuracy. Also, if you need to remove a larger amount of liquid outside the range, use a pipette .

The above pipette information is provided by Shanghai Bashu. For more information on pipettes , please click:

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In 2007, Shanghai Bayu ( ) Industrial Co., Ltd. established an offline supply system for laboratory instruments, MRO industrial products, etc. In 2009, it officially launched an online procurement operation platform.

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