Eyeliner,White Eyeliner,Nyx Eyeliner,Liquid Eyeliner Guangzhou Lingxue Cosmetics Co., Ltd , https://www.lxgj188.com
Application of GS-Smart Small Automatic Gel Dye Shaker in Coomassie Brilliant Blue Dyeing Experiment
Coomassie blue staining ( CBB staining ) is a fairly common method used in protein staining experiments. It overcomes the limitation of low sensitivity of amino black staining. It is one of the most sensitive protein assays, but it is better than silver nitrate. Other methods such as dyeing are easier and easier to operate, and are therefore widely used.
The full experimental process of Coomassie Brilliant Blue staining has two key and time-consuming steps, dyeing and discoloration. Typically, in order to allow the protein to fully gel staining and destaining, generally has a CBB staining solution was added to continue bleaching swing Shaker work tank, and then infiltrating into the gel washed sufficiently, thereby achieving bleaching stain. In addition to the adjustment of the swing frequency of the working pool, the ordinary decolorizing shaker can be set without other parameters. The steps of liquid inlet, liquid exchange and draining must be manually completed by the experimenter. Moreover, after the start-up, since the working pool generally continues to oscillate continuously, the dyeing and discoloring time can only be grasped by the experimenter himself. Therefore, the general Coomassie blue staining decolorization experiment generally requires the presence of an experimenter.
Further, for the immobilization of proteins in an acidic environment and maintained CBB before dyeing, but also to remove interfering substances remaining pre-stained electrophoresis, CBB staining solution or bleaching solution is generally in the configuration of methanol was added and sometimes strong irritant neurotoxic acetic acid, and with a good general population CBB staining was brownish-black (CBB R-250). Shaker ordinary work pool completely open exposure, it is possible during the swing spill overflow CBB staining solution, bleaching solution, it is also possible toxic volatile compounds. This is not only easy to cause pollution, but may even cause safety hazards, thereby jeopardizing the health of the experimental personnel.
Ding Haoyuan GS-Smart small automatic gel dyeing shaker (also known as automatic gel staining instrument) can well solve the problems faced by the above common Coomassie blue staining decolorization experiment, but also bring more convenience.
First of all, her intelligent programming function can realize the automatic operation of liquid inlet, liquid exchange, liquid discharge, timing shaking and waste liquid recovery. The whole process does not require personnel to be on duty, so that the CBB dyeing decolorization experiment is truly fully automated.
Secondly, she can be closed with a dyeing tank, can effectively prevent the CBB staining liquid, bleaching liquid spill overflow barrier volatile substances, thereby greatly reducing the risk of contamination, protecting the health of laboratory personnel. The dyeing tanks are available in a variety of sizes and can even be customized to meet as many different needs as possible for different scientific researchers' Coomassie blue staining experiments.
Thirdly, Dingyuanyuan GS-Smart small automatic gel dyeing shaker is also very simple and convenient to operate. It only needs four steps: “adding solution, placing gel, setting up pipeline program, clicking and runningâ€. It is easy to get the whole process of Coomassie blue dyeing and bleaching, saving time and effort.
In addition, it is worth mentioning that Dinghaoyuan GS-Smart small automatic gel dyeing shaker also has the world's first integrated fuselage and liquid storage bottle design, compared with similar products on the market, reducing the dispersion. Bottles and cans save lab space while also beautifying the overall look.
Overall, Dingyuan GS-Smart small automatic gel dyeing shaker is an instrument specially designed for automatic gel dyeing, which is very suitable for Coomassie blue staining and decolorization experiment. By replacing the traditional bleaching shaker, it is bound to set off a revolutionary wave of CBB dyeing and bleaching automation, which will bring satisfactory experimental results and convenient and pleasant experimental experience for the majority of researchers.