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First, the physical and chemical properties: ozone at room temperature is a blue explosive gas, a special odor, known as the strongest oxidant, a density of 1.658 (air = 1). Ozone gas can be liquid after cold treatment. Its liquid density is 1.71, and its boiling point is -112.3°C. Its solubility in water is higher than that of oxygen. However, due to the low partial pressure, it can only obtain the number of litres when the temperature and pressure are used normally. A solution of milligrams, a solution containing ozone, will explode when warmed. The stability of ozone is extremely poor. It can be decomposed to oxygen at room temperature and can be converted to oxygen immediately at a high temperature of 270°C. The half-life of 1% aqueous solution in atmospheric air is 16 minutes, so ozone cannot be stored in bottles as other industrial gases. It is generally produced on site and used immediately.
Second, the sterilization effect: ozone is a broad-spectrum fungicide, can kill bacteria and spores, viruses, fungi, etc., and can destroy botulinum toxin. Ozone kills bacteria faster than chlorine in water.
Third, the factors that affect the bactericidal effect:
1PH: When the pH is increased with the ozone water solution, the required concentration must be increased.
2 Humidity: When fumigated with ozone, the relative humidity is high, the effect is good, and the effect is low, and there is almost no bactericidal effect on the dried bacteria.
3 Temperature: The decrease of temperature is conducive to the dissolution of ozone, which can enhance its disinfection effect, even at 0 °C can maintain a good bactericidal effect, such as the water temperature of 4-6 °C, the ozone sterilization dose of 100, the water temperature of 10-21 °C When the water temperature is 36-38°C, it is 320, organic matter can reduce its bactericidal effect.
IV. Toxicity: When the concentration of ozone in the air reaches 0.01-0.02mg/L, it can be smelled: When the concentration reaches 1mg/L, it can cause symptoms such as accelerated breathing, change of blood pressure, and chest tightness, etc., at 2.5-5mg/L. Can cause pulse acceleration, fatigue, headache, stay emphysema for 1 hour, and even death, the allowable threshold in the air at the job site is 0.2mg/m3.
Fifth, corrosive: ozone is a strong oxidant, can damage a variety of items, the higher the concentration of the more damage to the item, can make the green rust appear on the copper, especially the rubber aging, darker color, reduced elasticity, resulting in brittle, Break, make the fabric bleach and fade.
6. Stability: The ozone stability is extremely poor, and it can be decomposed to oxygen at normal temperature. After the stoppage occurs, the concentration is the same as the atmospheric level after 30-60 minutes of ventilation.
VII. Scope of use: In terms of disinfection, the use of ozone is mainly the following: 1 Liquid disinfection: purification and disinfection of drinking water, industrial domestic sewage and beverage water.
2 Disinfection of surfaces, food and appliances, barber tools, food processing tools, clothing, coins, tickets, etc.
3. Anti-corrosion and preservation: vegetables, fruits, eggs, fish and meat, dry and fresh native products, aquatic product processing, storage and refrigeration, etc.
Eight, use: 1 liquid disinfection: ozone disinfection of drinking water, the amount depends on the quality of the water, should be determined by the experimental accuracy, more clean water, generally should add ozone 0.5-1mg/L, after 5-10 minutes, The concentration of ozone in the water is maintained at 0.1-0.5 mg/L; for drinking water with severe pollution, the amount of ozone can be increased to 3-6 mg/L. For sewage treatment, the concentration of ozone used in the sewage is 100-200 mg/L, and for 30 minutes, in most cases, all microorganisms and their toxins can be killed or destroyed, and water quality can be improved. The longer the effect, the better the effect.
2. Disinfect air: Apply air with a concentration of 5-10 mg/m3 to air in the dense space for 30 minutes.
Nine, matters needing attention: As ozone is a strong oxidant, the damage to the goods is relatively large, and is rarely used for fumigation, and 0.2 mg/m3 is allowed in the atmosphere. Therefore, disinfection should be carried out under unmanned conditions.
Ten, content determination: In a 500ml conical stoppered glass bottle, add 350ml distilled water and 20ml 20% KI solution, take the ozone gas 2L into the conical flask in the exhaust pipe, and then drop 5ml of 3mol/L H2SO4 solution , After standing for 5 minutes, titrate with 0.1000mol/L Na2S2O3. Add 1ml 0.5% starch indicator to pale yellow, titrate to colorless, calculate the consumed amount. Each ml/l of Na2S2O3 solution is equivalent to 48.00mg of O3.
O3 concentration (mg/L) = MV481000/22
100=12MV
In the formula: M = molar concentration, four digits after the decimal point should be calibrated;
V = consume milliliters.
When the O3 concentration is high, you can take 1 liter of ozonized gas, then the formula is O3 concentration (mg/L) = 24MV
Ministry of Health "Disinfection Technical Specifications" - Ozone
Ministry of Health "Disinfection Technical Specifications" - ozone ozone, also known as three sub-oxygen, molecular formula O3, molecular weight of 48.00.