Whether you're heading back to the gym after the easing of pandemic restrictions or you're continuing to invest in home exercise equipment, online classes or fitness subscriptions, it's a good opportunity to make sure that you have the best tools for muscle recovery in your arsenal to help you reach your fitness goals. To achieve true workout recovery, you'll need a blend of nutrition and physical manipulation, but you can also take advantage of high-tech recovery tools meant to minimize muscle soreness (including the hard-to-resist CBD-infused activewear). If you're looking for a workout tool with science on its side, consider a Massage Gun. Massagers for Pain Relief,Electric Massagers,Handheld Massagers,Hand Held Electric Massager Ningbo Shuangtuo International Trade Co., Ltd. , https://www.nbst-sports.com
Massage guns use the force of percussive therapy to manipulate your body's soft tissue. They're essentially backed by the same extensive scientific research that supports massage therapy as the optimal tool for sore muscles after a workout. Everyone from recreational gym-goers to professional athletes and people with chronic pain -- they all love these powerful massagers for many reasons.
Percussive therapy is said to help muscles recover faster while reducing muscle pain, muscle fatigue and lactic acid buildup. A percussion gun allows you to focus on a certain muscle group for immediate pain relief. They can also improve your range of motion and flexibility, encourage blood flow, help with muscle stiffness and more. Percussive therapy may even help with stress and sleep. Also, not that you should invest in one for this reason alone, but the slow-motion videos of massage guns punching muscles look insanely Insta-worthy. Just be careful with using one if you have any injuries beyond a muscle ache from a tough workout.
Differences between paraffin sections, frozen sections and vibrating sections
First, paraffin section
The repaired wax block is placed on a rotary microtome to perform sectioning. Slices must be kept sharp, the slice thickness is usually 5 ~ 7um, can also be cut into different thickness according to the dyeing needs, generally not rotating microtome more than 10um. Cut the wax strip and let the wax sheet flatten in warm water of about 40 °C. Good slicing should be free of knife marks and cracks, and the thickness of the slice should be flat. If the above problems occur in the sectioning, a false positive phenomenon occurs in immunohistochemical staining. In order to obtain a flat, non-pleated tissue section. Two pieces can be used, that is, the tissue slice is first floated in a 30% ethanol solution for the first time, then the slice is picked up, and then placed in a 45-50 ° C water bath for a second exhibition. The concentration of ethanol and the temperature of the water are adjusted independently depending on the tissue and the melting point of the wax. This process uses the difference in tension between the ethanol solution and water to unfold the wrinkles of the slice. To prevent stripping, slides should be coated with a tablet. For HE-stained sections, thin layer of protein glycerol can be applied to the slides, but protein glycerol is easy to cause non-specific immune response due to protein content, so the sections used for immunohistochemical staining are usually polylysine, 3-amino-propionate. Treatment with keto-ethoxysilane or the like.
Second, frozen slices
The frozen section (fiozen section) is the most commonly used sectioning method in enzymatic histochemistry and immunohistochemical staining. Its most prominent advantage is that it can preserve the surface of the cell membrane and the various enzyme activities in the cell and the immunological activity of the antigen. In particular, cell surface antigens should be frozen. Fresh tissue and fixed tissue can be frozen. In order to obtain high-quality frozen slices, the selection of a good frozen slicer and the matching disposable knife holder is the key to ensure the quality of the slice. The tissue needs to be frozen before slicing, and the freezing process tends to cause ice crystals in the tissue to form ice crystals, thereby affecting antigen localization. It is generally believed that when the volume of ice crystals is large and the amount is small, the influence is small; when the volume of ice crystals is small and the amount is large, the damage to the structure is large. Ice crystals are more likely to occur in tissues with more water content.
1. Method for preventing formation of ice crystals in tissues
(1) Quick freezing: The temperature of the tissue is drastically reduced to reduce the formation of ice crystals. Methods include:
1) Dry ice-acetone (ethanol) method: 150-200ml of acetone (anhydrous ethanol) is placed in a small vacuum flask, and gradually added to the dry ice until the saturation is viscous. When the dry ice is no longer bubbling, the temperature can reach - At 70 ° C, about 50 ml of isopentane was placed in a small beaker. The beaker was slowly placed in a dry ice-acetone (or absolute ethanol) saturated solution, and the isopentane temperature was -70 ° C. The tissue (size lcm×0.8 cm×0,5 cm) was placed in isopentane for 30-60 seconds, and then taken out, placed in a constant cold box for slicing, or stored in a low-temperature refrigerator at -80 °C.
2) Liquid nitrogen method: place the tissue block flat on a soft plastic bottle cap or a special tin foil box (about 2 cm in diameter), and add the OCT embedding agent to the tissue in an appropriate amount, and then slowly place the special small box to hold the liquid. In the small cup of nitrogen, when the bottom of the box contacts liquid nitrogen, it begins to vaporize and boil. At this time, the small box remains in place, and it is not immersed in liquid nitrogen. After about 10 to 20 seconds, the tissue quickly freezes into a block. The frozen tissue pieces were taken out and immediately placed in a cryostat slicer for frozen sectioning or placed in a -80 ° C refrigerator for storage.
(2) Application of sucrose solution: The tissue is placed in a 20% to 30% sucrose solution for 1-3 days, and the water in the tissue is absorbed by hyperosmosis to reduce the water content of the tissue, thereby preventing or reducing the formation of ice crystals.
2. Constant cold box slicing
The frozen tissue block can be used for cryostat sectioning, and the process includes the following steps:
(1) Tissue embedding According to the research purpose, take the required tissue (such as cutting the mouse liver 1.0cm × l.0cm × 0.5cm size tissue block), use filter paper to remove excess water, and put the tissue block flat. Special tin foil box (about 2cm in diameter) or soft plastic bottle cap. Add appropriate amount of OCT embedding agent to immerse the tissue and soak for 20-30 minutes at 40 °C. To prevent the formation of ice crystals, sucrose treatment can be carried out before embedding.
(2) Tissue freezing: The tissue is frozen by the above dry ice-acetone (ethanol) method or liquid nitrogen method.
(3) Sectioning: The temperature of the cryostat slicer was set to about -18 °C 1 hour before sectioning to prepare for slicing. When slicing, the temperature is preferably -15--18 ° C. If the temperature is too low, the tissue is easily broken. The angle of the knife should be appropriate? Otherwise it is not easy to connect. Do not move up and down when attaching tissue sections with slides. Fresh tissue that has not been fixed should be fixed with the appropriate fixative for 10 minutes after frozen sectioning. If it is not immediately stained after frozen section, it must be blown dry with an electric fan, stored in a -70 ° C low temperature refrigerator or temporarily pre-fixed and stored in a low temperature refrigerator. Remove the sections from the refrigerator before dyeing, leave them to dry at room temperature for 10 minutes, and then fix them in cold acetone for 5 to 10 minutes (unfixed). After rinsing 3 times with PBS, you can enter the staining procedure (HE staining can be used with formaldehyde, glacial acetic acid and 95%). Ethanol is quickly fixed for 1-2 minutes).
(4) Preservation of frozen slices: If it is not stained in time after freezing, it can be placed in a sealed specimen box after drying, wrapped in a plastic bag, stored in a low-temperature refrigerator, or temporarily pre-fixed and stored in a low-temperature refrigerator.
Third, vibration slice
Vibrating microtome (the vibratome) may be fresh tissue (not fixed, not frozen) cut into thick slices 20 ~ 400pm to flotation histochemical or immunohistochemical staining in the plate. Because the tissue is not frozen, no ice crystals are formed and no tissue antigen is destroyed. Before the dyeing, the steps of tissue dehydration, transparency, embedding and the like are avoided, so that the fat-soluble substances and cell membrane antigens in the tissue can be well preserved. Vibration sections are mainly used to show the study of the distribution of antigens in the nervous system. For soft embryonic brain tissue, it can be embedded in 10% agarose with low melting point (45-55 ° C), cut into thick slices (40-100 gm) by vibrating slicer, and immunofluorescence histochemical staining in the reaction plate by floating method. Laser scanning confocal microscopy.